ABSTRACT
Objective: To detect the peri-implant soft tissue with Florida probes, and to provide the clinical basis for choosing the appropriate clinical examination method, probing strength, and treatment of peri-implant soft tissue in the maintenance period. Methods: The common periodontal probes and Florida probes were used to examine the probing depth (PD) values in 62 patients who underwent implantation for more than 6 months for two times, and the contralateral teeth were natural teeth. The natural teeth and the implants were divided into inflammation group (n=32) and healthy group (n=30) according to whether the bleeding was probed. The coefficient of variation (CV) values of PD values of natural teeth and peri-implant soft tissues detected by two probes were compared. Results: There were no statistically significant differences in the PD values between natural teeth and implants of the patients measured by common periodontal probes in healthy and inflammation groups (P> 0. 05). There were no statistically significant differences in the PD values between natural teeth and implants of the patients measured by Florida probes in healthy and inflammation groups (P>0. 05). The CV value of PD value detected by Florida probes of the patients in healthy group was less than that detected by common periodontal probes (t=2. 489, P=0. 019); the CV value of PD value detected by Florida probes of the patients in inflammation group was less than that detected by common periodontal probe (t=2. 238, P = 0. 033). The CV value of PD value of implants of the patients in inflammation group was higher than that in healthy group (Z=3. 804, P0. 05). Conclusion: Both two probes have good reproducibility in probing the healthy and inflammatory sites of the natural teeth and implants. The Florida probes are more reproducible than the common periodontal probes in probing the healthy and inflammatory peri-implant soft tissues.
ABSTRACT
<p><b>OBJECTIVE</b>This paper aimed to determine the mRNA expression of osteoclast-related factors interleukin-6 (IL-6), interleukin-12 (IL-12) p35, IL-12p40, matrix metalloproteinase-9 (MMP-9), nuclear factor of activated T-cells cytoplasmic 1 (NFATcl), receptor activator of nuclear factor-KB (RANK), and tumor necrosis factor-α (TNF-α) mRNA in murine macrophages infected by a periodontitis patient's own tissue nucleic acid. Another aim was to investigate the effects of a periodontitis patient's own tissue nucleic acid on the differentiation of macrophages into osteoclasts.</p><p><b>METHODS</b>Inflammatory periodontal tissue samples of chronic periodontitis patients were taken during periodontal flap surgery, and healthy gingival tissue samples were taken from orthodontic patients during tooth extractions. Total RNA from periodontal tissue was extracted and reversely transcribed into cDNA and then cryo-preserved until further use. First, specific sequence oligodeoxynucleotide MT0I at a concentration of 1 µg · mL⁻¹ was added in murine macrophage RAW264.7, and the cells were incubated for 3 hours. Cells with PBS (1 µg · mL⁻¹) were used as negative controls. The inflammatory periodontal tissue cDNA and healthy periodontal tissue cDNA (1 µg · mL⁻¹) was added subsequently. There were four experimental groups: healthy periodontal tissue cDNA+ RAW264.7, inflammatory periodontal tissue cDNA+RAW264.7, MT01+healthy periodontal tissue cDNA+RAW264.7, and MT01+inflammatory periodontal tissue cDNA+RAW264.7. Real-time quantitative polymerase chain reaction was used to detect the mRNA expression of osteoclast-related factors IL-6, IL-12p35, IL-12p4O, MMP-9, NFATcl, RANK, and TNF-α mRNA after 3, 6, 12, and 24-hours.</p><p><b>RESULTS</b>The mRNA levels of osteoclast-related factors NFATc1, MMP-9, TNF-a, IL-6, IL-12p40, IL-12p35, and RANK in RAW264.7 were markedly upregulated with the treatment of periodontitis patient's own tissue nucleic acid. However, the mRNA expression of osteoclast-related factors was inhibited by use of an immunosuppressant MT01.</p><p><b>CONCLUSION</b>The periodontitis patient's own tissue nucleic acid could promote the differentiation of murine macrophage into osteoclasts.</p>
Subject(s)
Animals , Humans , Mice , Cell Differentiation , Cytokines , Metabolism , Gene Expression , Gingiva , Interleukin-12 Subunit p40 , Interleukin-6 , Macrophages , Matrix Metalloproteinase 9 , Osteoclasts , Metabolism , Periodontitis , RNA, Messenger , Tumor Necrosis Factor-alphaABSTRACT
A technique of solid-phase pla-telet immunoserological assay wasused to select unsuitable plateletdonors for patients with refractory (?)diopathic thrombocytopenic purpura(RITP).The unsuitable plateletswere used as carriers to bind with VP-16 (Etoposide),and then wesetransfused into5patients with RITP.All the cases were benefited by sucha therapy,and 4 of them were cured.indicating that the therapy has itscurative value in clinical practice.(Original article on page 115)